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Copyright © 2000 Cell Press. All rights reserved.
Molecular Cell, Volume 6, Issue 2, 395-407, 1 August 2000
doi:10.1016/S1097-2765(00)00039-3
Article
The Protooncogene TCL1 Is an Akt Kinase Coactivator
Jarmo Laine1, Gerald Künstle1, Toshiyuki Obata2, Ma Sha3 and Masayuki Noguchi1, §, *, 
1 Department of Medicine, Division of Immunology, Beth Israel Deaconess Medical Center and, Harvard Medical School , Boston, Massachusetts 02215, USA
2 Department of Medicine, Division of Signal Transduction, Beth Israel Deaconess Medical Center and, Harvard Medical School , Boston, Massachusetts 02215, USA
3 Ciphergen Biosystems, Incorporated, Palo Alto, California 94306, USA
Corresponding author: Masayuki Noguchi, 617 632 0521 (phone), 617 632 0160 (fax)Summary
Human T cell prolymphocytic leukemia can result from chromosomal translocations involving 14q32.1 or Xq28 regions. The regions encode a family of protooncogenes (TCL1, MTCP1, and TCL1b) of unknown function. In yeast two-hybrid screening, we found that TCL1 interacts with Akt. All TCL1 isoforms bind to the Akt pleckstrin homology domain. Both in vitro and in vivo TCL1 increases Akt kinase activity and as a consequence enhances substrate phosphorylation. In vivo, TCL1 stabilizes the mitochondrial transmembrane potential and enhances cell proliferation and survival. In vivo, TCL1 forms trimers, which associate with Akt. TCL1 facilitates the oligomerization and activation of Akt. Our data show that TCL1 is a novel Akt kinase coactivator, which promotes Akt-induced cell survival and proliferation.
