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- CDK Activity Antagonizes Whi5, an Inhibitor of G1/S Transcri...CDK Activity Antagonizes Whi5, an Inhibitor of G1/S Transcription in Yeast
Cell, Volume 117, Issue 7, 25 June 2004, Pages 899-913
Michael Costanzo, Joy L Nishikawa, Xiaojing Tang, Jonathan S Millman, Oliver Schub, Kevin Breitkreuz, Danielle Dewar, Ivan Rupes, Brenda Andrews and Mike Tyers
SummaryCyclin-dependent kinase (CDK) activity initiates the eukaryotic cell division cycle by turning on a suite of gene expression in late G1 phase. In metazoans, CDK-dependent phosphorylation of the retinoblastoma tumor suppressor protein (Rb) alleviates repression of E2F and thereby activates G1/S transcription. However, in yeast, an analogous G1 phase target of CDK activity has remained elusive. Here we show that the cell size regulator Whi5 inhibits G1/S transcription and that this inhibition is relieved by CDK-mediated phosphorylation. Deletion of bypasses the requirement for upstream activators of the G1/S transcription factors SBF/MBF and thereby accelerates the G1/S transition. Whi5 is recruited to G1/S promoter elements via its interaction with SBF/MBF in vivo and in vitro. In late G1 phase, CDK-dependent phosphorylation dissociates Whi5 from SBF and drives Whi5 out of the nucleus. Elimination of CDK activity at the end of mitosis allows Whi5 to reenter the nucleus to again repress G1/S transcription. These findings harmonize G1/S control in eukaryotes.
Summary | Full Text | PDF (838 kb) - Periodic Transcription: A Cycle within a CyclePeriodic Transcription: A Cycle within a Cycle
Current Biology, Volume 13, Issue 1, 8 January 2003, Pages R31-R38
Linda L Breeden
SummaryStudies in model organisms indicate that one in every five genes may be subject to cell cycle regulated transcription. Moreover, a high proportion of periodically expressed genes have discrete roles in the cell division process, and their peaks of expression coincide with the interval during which they function. This periodic transcription is commonly regulated by transcription factors that are also periodically transcribed, and there is a growing number of examples where the transcription factors and their targets are conserved in yeast and mammalian cells. As such, it is worth considering why these regulatory circuits persist in such great number, how they are achieved and what role they may play in the cell cycle.
Summary | Full Text | PDF (146 kb) - Forkhead transcription factors, Fkh1p and Fkh2p, collaborate...Forkhead transcription factors, Fkh1p and Fkh2p, collaborate with Mcm1p to control transcription required for M-phase
Current Biology, Volume 10, Issue 15, 1 August 2000, Pages 896-906
Raman Kumar, David M Reynolds, Andrej Shevchenko, Anna Shevchenko, Sherilyn D Goldstone and Stephen Dalton
SummaryForkhead transcription factors have redundant roles in the control of CLB2 cluster genes during the G2–M period of the cell cycle, in collaboration with Mcm1p.
Summary | Full Text | PDF (330 kb) - …more
Copyright © 1998 Cell Press. All rights reserved.
Molecular Cell, Volume 2, Issue 1, 65-73, 1 July 1998
doi:10.1016/S1097-2765(00)80114-8
Article
A Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle
Raymond J. Cho1, #, Michael J. Campbell2, #, ∣∣, *, 
, Elizabeth A. Winzeler2, Lars Steinmetz1, Andrew Conway2, Lisa Wodicka3, Tyra G. Wolfsberg4, Andrei E. Gabrielian4, David Landsman4, David J. Lockhart3 and Ronald W. Davis1, 2
1 Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
2 Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA
3 Affymetrix, 3380 Central Expressway, Santa Clara, California 95051, USA
4 National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland 20894, USA
Corresponding author: Michael J. Campbell, 650 723 6161 (phone), 650 723 6783 (fax)Summary
Progression through the eukaryotic cell cycle is known to be both regulated and accompanied by periodic fluctuation in the expression levels of numerous genes. We report here the genome-wide characterization of mRNA transcript levels during the cell cycle of the budding yeast S. cerevisiae. Cell cycle–dependent periodicity was found for 416 of the 6220 monitored transcripts. More than 25% of the 416 genes were found directly adjacent to other genes in the genome that displayed induction in the same cell cycle phase, suggesting a mechanism for local chromosomal organization in global mRNA regulation. More than 60% of the characterized genes that displayed mRNA fluctuation have already been implicated in cell cycle period-specific biological roles. Because more than 20% of human proteins display significant homology to yeast proteins, these results also link a range of human genes to cell cycle period-specific biological functions.
